GM508 CultActive

GM508 CultActive is a bicarbonate-buffer­ed reagent designed to investigate oocytes of patients with failed fertilization after previous Intracytoplasmatic Sperm Injection cycles.

GM508 CultActive is designed to investigate if fertilization failure after previous ICSI-cycles is due to a deficient oocyte activation.

Product description Product code Unit
CultActive 4 GM 508CULT-active1 1 ml
  • GM508 CultActive must be shaken directly before use for approximately 30 sec.
  • GM508 CultActive must be equilibrated 4 hours in a vial not firmly closed at 5-7 % CO2 and 37°C prior to use.
  • Equilibrate culture medium for washing (e.g. GM501 Cult) for 4 hours in a vial not firmly closed at 5-7 % CO2 and 37°C prior to use.
  • Prepare for each oocyte 1 drop (30 µl) GM508 CultActive and 2 drops (30-50 µl) MOPS- and HEPES-free culture medium, (e.g. GM501 Cult). An oil overlay is not necessary due to the short exposure time.
  • Immediately after the ICSI procedure incubate the oocytes for 15 minutes in the pre-equilibrated Ca2+-Ionophore GM508 CultActive drops. -Step 1
  • Remove the oocytes from the GM508 CultActive drop and wash them twice in culture media. This has to be done in a HEPES-or MOPS-free medium, e.g. GM501 Cult. -Step 2 and 3
  • Put the oocytes in your culture media for further culture.
  • Assess the development on specific time points.

Brand

Gynemed

Gynemed has been dedicated to the development, manufacture and distribution of high-quality medical products for reproductive medicine since 1998. In our portfolio you will find exclusively products meeting the highest standards and demands on quality, representing the latest scientific developments.

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