GM501 SpermStore 20 ml

Instructions for use:

• Ensure all media are well mixed before use.

Before freezing:

• In case of very low sperm concentrations it is advisable to concentrate the sperm before freezing. In case of very high sperm concentration, sperm can be diluted using a suitable sperm washing medium.
• GM501 SpermStore needs to be warmed to room temperature before use to avoid cold-shock.

Freezing:

1. Allow the semen to liquefy at room temperature for 30 minutes.
2. Mix 1 ml of sperm with 0.7 ml of GM501 SpermStore. Add the medium in drops while gently swirling.
3. Leave the mixture for 10 minutes at room temperature for equilibration.
4. Aspirate the sample/medium mixture into the freezing straws, leaving approximately 1.5 cm of air at the end of the straws.
5. Seal the straws.
6. Dry off individually with a lint-free wipe.
7. Shake to move the air-bubble to the centre of the straw.
8. Freeze horizontally for 15 minutes, just above the level of the liquid nitrogen.
9. Store in liquid nitrogen.

Thawing:

1. Remove as many straws as required from the liquid nitrogen.
2. Place the samples in tap water for 5 minutes (room temperature or 37°C).
3. Cut off the end of the straw, place the open end inside a container (e.g. a test tube) and tap the straw lightly against the side of the container to allow complete evacuation of the mixture.
4. Dilute the thawed sperm in a suitable insemination medium (at least 3 ml per 0.5 ml semen) and mix thoroughly.
5. Centrifuge for 15 minutes at 300-350 x g.
6. Resuspend pellet in a suitable insemination medium.

After thawing:

• If necessary, use sperm preparation techniques after thawing the semen to eliminate dead sperm cells and debris. Dilute the concentrated sperm in a suitable insemination medium.

Pre-use checks:

• • Do not use the product if bottle, seal of the container or package is opened or defect when the product is delivered.
  • Do not use the product if it becomes discoloured, cloudy or shows any evidence of microbial contamination.