GM501 Hyaluronidase 5 x 1 ml

Instructions for use:

• GM501 Hyaluronidase contains HEPES; no CO₂ incubation is required, just warm it up to 37°C (incubation in a CO₂ incubator will lower the pH below 7).
• Depending on the dishes, 100 µl (micodrops) or 400-600 µl (center-/4-well) of GM501 Hyaluronidase should be used for denudation.
• Additionally prepare 3-5 drops (100 µl) of washing medium (e.g. GM501 Wash) for oocyte washing.
• The dishes should be covered with Mineral Oil (e.g. GM501 Mineral Oil).
• Place the oocyte in the Hyaluronidase (up to max. 5 oocytes) for about 30 seconds.
• Using a fine pipette, transfer the partially denuded oocytes to the first washing drop.
• Remove the corona by repeatedly pipetting the oocytes.
• Use the other drops to further wash the denuded oocyte.

Instructions for use:

• GM501 Hyaluronidase contains HEPES; no CO₂ incubation is required, just warm it up to 37°C (incubation in a CO₂ incubator will lower the pH below 7).
• Depending on the dishes, 100 µl (micodrops) or 400-600 µl (center-/4-well) of GM501 Hyaluronidase should be used for denudation.
• Additionally prepare 3-5 drops (100 µl) of washing medium (e.g. GM501 Wash) for oocyte washing.
• The dishes should be covered with Mineral Oil (e.g. GM501 Mineral Oil).
• Place the oocyte in the Hyaluronidase (up to max. 5 oocytes) for about 30 seconds.
• Using a fine pipette, transfer the partially denuded oocytes to the first washing drop.
• Remove the corona by repeatedly pipetting the oocytes.
• Use the other drops to further wash the denuded oocyte.