GYNEMED GM501 PVP

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A ready-to-use 10% dialysed PVP medium to reduce sperm motility, facilitating capture of spermatozoa for ICSI.

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Brand: Gynemed
Detalis: GYNEMED GM501 PVP is a 10 % dialyzed polyvinylpyrrolidone medium designed specifically to reduce sperm motility, making it easier for embryologists to capture individual spermatozoa for ICSI procedures. ([turn0search0][turn0search1]) Composition & Specifications Contains dialyzed PVP (10 %) of European Pharmacopoeia grade dissolved in an aqueous solution that includes physiological salts, HEPES buffer, lactate, pyruvate, glucose, and human serum albumin (~4.00 g/L). Gynemed +2 Gynemed +2 pH (37 °C): 7.20 – 7.90 (release criteria: 7.20 – 7.60) Gynemed +2 Gynemed +2 Osmolality: 280 – 320 mOsm/kg Gynemed +2 Planer +2 Sterility: sterile, SAL = 10⁻³ Gynemed +1 Endotoxin level: < 1.00 EU/mL Gynemed +2 Gynemed +2 Viscosity (25 °C): > 170 cP (centipoise) Gynemed +1 MEA performance: ≥ 80 % of blastocysts after 96 hours when sperm are exposed for 10 min to PVP medium. Gynemed +1 Usage / Protocol Warm the PVP medium to 37 °C before use. Gynemed +1 Place a small drop (5-10 μL) of PVP solution in a dish, overlay with GM501 MineralOil. Gynemed +1 Add a small volume (1-2 μL) of washed spermatozoa to the centre of the PVP droplet. Gynemed +1 Wait a few minutes to allow sperm to migrate toward the edge of the droplet. Gynemed +1 Select the sperm and collect them for injection (ICSI). Gynemed +1 Storage & Stability Shelf life: 9 months from manufacture. Gynemed +1 Storage temperature: 2-8 °C. Do not freeze. Keep away from light. Gynemed +1 After opening: Safe to use up to 7 days under sterile conditions if kept at 2-8 °C. Gynemed +1 Precautions & Warnings Contains human serum albumin (a blood derivative). Although processed to minimize risk, all human-derived materials should be treated as potentially infectious. Gynemed +1 The product does not contain antibiotics; aseptic technique is essential. Gynemed +1 If the product is discolored, cloudy, or container is damaged — do not use. Gynemed +1